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Transfer

Transfer buffer ensures efficient and uniform migration of proteins or nucleic acids from gels to membranes during blotting procedures like Western, Southern, and Northern blotting. It maintains consistent pH and ionic strength throughout the transfer process, helping preserve target molecule integrity and maximizing signal clarity.

  • Provides stable pH and ionic environment for effective blot transfer
  • Supports protein and nucleic acid transfer from SDS-PAGE, Native PAGE, and agarose gels
  • Compatible with PVDF and nitrocellulose membranes for western, southern, or northern blotting

Whether you're transferring proteins for antibody detection or nucleic acids for hybridization, our transfer buffer delivers reliable performance and sharp, reproducible results.

Transfer

Showing 1–3 of 3 products

Transfer Buffer (10X, Electro Blotting)

Transfer Buffer (10X, Electro Blotting)

From: $26.00

Semi Dry Blot Transfer Buffer (10X)

Semi Dry Blot Transfer Buffer (10X)

From: $31.00

Bis-Tris Transfer Buffer (20X)

Bis-Tris Transfer Buffer (20X)

From: $120.00

FAQs

Most transfer buffers include Tris (for pH control), glycine (for ionic strength), and optionally methanol, which improves protein binding to membranes and removes residual SDS.

Yes. While the composition may vary slightly, transfer buffers can support both protein and nucleic acid transfer in techniques like Western, Southern, and Northern blotting.

Buffers for SDS-PAGE often include methanol and SDS to support denatured protein transfer, while native PAGE buffers avoid harsh detergents to preserve protein structure.

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    Featured Products

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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