A qPCR buffer is formulated to ensure optimal amplification efficiency, sensitivity, and reproducibility in quantitative PCR workflows. It creates the precise chemical environment needed for robust DNA polymerase activity and accurate quantification of target sequences—even in low-abundance or complex samples.
Provides consistent pH, salt concentration, and cofactor balance for high-performance amplification
Enhances primer binding and enzyme stability throughout thermal cycling
Compatible with both DNA and RNA targets in SYBR Green, probe-based, and multiplex qPCR assays
Whether you're performing gene expression profiling, SNP genotyping, or viral load quantification, our qPCR buffer products deliver the precision your results depend on.
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Frequently Asked Questions
What is a qPCR buffer used for?
A qPCR buffer supports the amplification and detection of target DNA or RNA in real-time PCR by maintaining optimal pH, salt levels, and cofactor availability for the DNA polymerase.
What are the main components of a qPCR buffer?
Typical components include Tris (for pH control), KCl or MgClâ‚‚ (to support enzyme activity), and additives like DMSO or glycerol to stabilize the reaction and improve amplification of difficult templates.
Does buffer composition affect qPCR efficiency?
Absolutely. pH, magnesium concentration, and additives can significantly impact amplification efficiency, specificity, and sensitivity, especially in low-copy or GC-rich targets.
