Imidazole buffer is a nitrogen-rich heterocyclic compound solution widely used in protein purification, particularly for eluting histidine-containing proteins from divalent cation resins during immobilized metal affinity chromatography (IMAC). With an effective buffering range between pH 6.2 and 7.8, it ensures optimal conditions for various biochemical applications.
Provides precise control over protein binding and elution in affinity chromatography
Minimizes non-specific binding, enhancing the purity of target proteins
Ready-to-use formulation streamlines workflows, reducing preparation time and variability
Whether you're working on recombinant protein purification, enzyme assays, or other molecular biology applications, our imidazole buffer delivers consistent performance and reliable results.
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Frequently Asked Questions
What is imidazole buffer used for?
Imidazole buffer is commonly used in protein purification processes, especially for eluting His-tagged proteins in immobilized metal affinity chromatography (IMAC).
Why choose imidazole buffer over other buffers?
Imidazole buffer offers excellent control over protein binding and elution, reducing non-specific interactions and improving the purity of target proteins.
Can imidazole buffer be used in enzyme assays?
Yes, imidazole buffer is suitable for enzyme assays, providing a stable pH environment that supports enzyme activity.
