Imidazole Buffer (1 M, pH 7.0)
CAT#: BB-1117
- Useful in protein research, biochemistry, and molecular biology applications
- Effective in purification of histidine-tagged proteins, and within immunohistochemistry applications
- Ready-to-use solution, providing a buffering system within the pH range of 6.2-7.8
Available sizes:
Product Description
The Imidazole Buffer is an aromatic heterocyclic diazole with a buffering pH range of 6.2 to 7.8. It holds applications in molecular biology, biochemistry, and proteomics. It can be employed as a component in buffers for horseradish peroxidase assays (HRP assays), as well as a chelator for binding various divalent cations. Additionally, Imidazole aids in the elution of histidine-containing proteins from divalent cation resins, employing immobilized metal affinity chromatography (IMAC).
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 7.00 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Imidazole | 288-32-4 | 68.08 | 1 M |
| Hydrochloric Acid (6 N) | 7647-01-0 | 36.46 | For pH adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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Imidazole Buffer (0.5 M, pH 7.0)- BB-2114Sku#: BB-2114