Hybridization buffer is designed to create the optimal environment for nucleic acid probe binding in applications like in situ hybridization and nucleic acid hybridization. Its precise formulation promotes high-specificity hybridization while reducing background noise, ensuring accurate detection of target DNA or RNA sequences.
Maintains optimal pH and ionic strength to support specific probe-target binding
Reduces non-specific interactions for clear, high-sensitivity results
Compatible with DNA and RNA workflows including blots, microarrays, and ISH
Whether you're mapping gene expression or detecting viral RNA, our hybridization buffers provide the reliability and sensitivity your nucleic acid assays require.
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Frequently Asked Questions
What is a hybridization buffer used for?
A hybridization buffer facilitates the binding of complementary nucleic acid probes to their target DNA or RNA during techniques such as in situ hybridization and blotting assays.
What are common components in a hybridization buffer?
These buffers typically contain salts (e.g., sodium chloride or phosphate), detergents (e.g., SDS), and sometimes formamide or dextran sulfate to control stringency and reduce non-specific binding.
How does hybridization buffer improve sensitivity in assays?
By controlling ionic strength and including blocking agents, hybridization buffers minimize non-specific interactions, enhancing the signal-to-noise ratio and improving detection sensitivity.
