Gel running buffer plays a critical role in electrophoresis by providing the stable pH and ionic strength needed to ensure consistent migration of proteins or nucleic acids through the gel matrix. This buffer supports consistent biomolecule separation and sharp band resolution across a range of molecular weights in both SDS-PAGE and native PAGE systems.
Ensures stable pH and ionic strength for accurate molecular separation
Supports DNA, RNA, and protein electrophoresis workflows
Compatible with systems such as SDS-PAGE, native PAGE, and Tris-Glycine-based protocols
Whether you're working with nucleic acids or proteins, our gel running buffer delivers reproducible results and dependable performance across your electrophoresis experiments.
Read More
Frequently Asked Questions
What is a gel running buffer used for?
A gel running buffer is used during electrophoresis to carry electric current and maintain pH, allowing biomolecules like DNA, RNA, or proteins to migrate consistently through the gel.
What signs indicate that a gel running buffer should be replaced?
Discoloration, cloudiness, or a drop in conductivity may indicate degradation or contamination. Also, poor band resolution or unexpected migration can signal an issue with the buffer.
What's the difference between Tris-Glycine and Tris-Tricine buffers?
Tris-Glycine is widely used for standard SDS-PAGE, while Tris-Tricine is better suited for resolving small proteins or peptides below ~10 kDa.
