CHAPS lysis buffer is a zwitterionic, non-denaturing solution designed to solubilize membrane proteins while preserving their structure and interactions. It’s ideal for applications requiring gentle, yet effective lysis, especially when maintaining protein activity or complex formation is critical.
Effectively solubilizes membrane and cytosolic proteins without disrupting function
Maintains protein conformation for co-IP, pull-downs, and enzyme activity assays
Suitable for native protein extraction in proteomics, structural biology, and receptor studies
Whether you're analyzing protein-protein interactions or isolating delicate membrane-bound complexes, our CHAPS buffer offers the balanced strength and specificity your protocols demand.
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Frequently Asked Questions
What is CHAPS lysis buffer used for?
CHAPS lysis buffer is used to extract proteins—especially membrane proteins—under non-denaturing conditions. It’s ideal for applications where preserving native structure and protein-protein interactions is important.
Can CHAPS buffer be used in enzyme assays?
Yes. Its mild nature allows enzyme activity to be retained, making it suitable for functional assays and activity-based profiling.
Can CHAPS buffer be used with sensitive cell types or organelles?
Yes, its non-denaturing nature makes it suitable for lysing delicate cells or isolating intact subcellular components like mitochondria or nuclei.
