Antibody-antigen docking: specificity lies in the details
A variety of common analytical methods relies on antibody-antigen interactions to highlight the presence of specific proteins in biological tissues. However, lengthy, multistep experiments have higher risks of generating false-positive and negative outcomes, resulting in inaccurate data.
Preparatory conditions, including the choice of buffers and reagents, influence the antibody's ability to reach and recognize the antigen of interest. Dehydration solvents, contaminants, or a poor choice of dilution buffers can significantly alter antigen retrieval, causing unreliable results and waste of biological resources.
The selection, manufacturing, and use of IHC Buffers are essential to ensuring successful target protein detection and highly reproducible results.
How does immunohistochemistry work?
Immunohistochemical staining highlights a specific protein or structure, based on labeled antibodies targeting an antigen of interest. Choosing the optimal IHC buffers, antibodies, and incubation times is critical for avoiding false-positive staining, and ensure proper visualization and long-term storage of your samples.
How we simplify the process
Detection of target protein biomarkers in tissue-a critical task, but often a challenge. Our team of experts and high-quality analytical-grade IHC Buffers help you get clear signals, even for tricky proteins, and in the correct cell type, subcellular location, and other spatial parameters.
Ways Boston BioProducts can help
Assure quality & traceability of IHC buffers
Research heavily depends on trust between scientists and lab suppliers. Can you trust yours? Directly manufactured in our ISO:13485 certified GMP facility, all our IHC Buffers and reagents follow a strict production process to minimize contamination risks, background signals, and lot variations.
Remain IHC-ready with near-on-demand buffer supply
With long-term storage for customer products, we make sure your IHC Buffers are ready when you need them and enable batch-to-batch consistency, allowing you to obtain reproducible results from your 1st to your 1000th IHC assay.
Efficiently troubleshoot with expert IHC support
Advocates of communication, trust, and transparency, we respond promptly to any question you may have. Need more certainty around your protocol for fixation, antigen retrieval, blocking, or visualization? Our specialists offer personalized technical advice to ensure you achieve specific detection and spatial accuracy.
Learn more about how
Boston BioProducts can help
Featured Products for Immunohistochemistry
Sample Preparation, Fixation and Embedding
Washing
Blocking of Nonspecific Activity
Featured Services & Resources
Quality Assurance
ISO 13485:2016 certified, our purpose-built facility follows a strict manufacturing process to guarantee customers' satisfaction and safety.
Certificate Request
We offer quality documentation, including Safety Data Sheets and Certificate of Analysis for all of our products upon request.
Order Support
Order your IHC Buffers today through our online catalog. Need some guidance? Our team is here for you!
Related Applications
ELISA
FPLC Protein Purification
Frequently Asked Questions
At Boston BioProducts, we want to make sure that you get it right the first time. Our specialists are available to guide you through your selection process, offering personalized advice tailored to your specific needs.
Reach out to one of them now and get an answer within hours.
We make a point of having our products readily available to guarantee their delivery in a timely manner. Our in-house manufacturing capabilities also allow our specialists to conceive and deliver custom buffers to your lab in days to weeks, minimizing production time and shipping delays. Learn more about our facilities.