Gel casting buffers are essential for forming the gel matrix used in electrophoretic separation of biomolecules. These buffers maintain consistent pH and ionic strength during gel polymerization, supporting reproducible migration and sharp resolution across applications like SDS-PAGE.
Supports consistent gel formation for reproducible band separation
Maintains ideal pH and conductivity during polymerization and electrophoresis
Compatible with a range of acrylamide gels
Our gel casting buffers deliver reliability from start to finish for polyacrylamide gel casting.
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Frequently Asked Questions
What is a gel running buffer used for?
A gel running buffer is used during electrophoresis to carry electric current and maintain pH, allowing biomolecules like DNA, RNA, or proteins to migrate consistently through the gel.
What signs indicate that a gel running buffer should be replaced?
Discoloration, cloudiness, or a drop in conductivity may indicate degradation or contamination. Also, poor band resolution or unexpected migration can signal an issue with the buffer.
What's the difference between Tris-Glycine and Tris-Tricine buffers?
Tris-Glycine is widely used for standard SDS-PAGE, while Tris-Tricine is better suited for resolving small proteins or peptides below ~10 kDa.
