HEPES Buffered Saline

HEPES Buffered Saline (HBS) is a versatile buffer solution designed to maintain stable, physiological pH (6.8–8.2) across a range of biological and biochemical applications. Containing HEPES as the primary buffering agent, HBS is less temperature-sensitive than Tris-based buffers, making it a reliable choice for sensitive experimental workflows.

HBS is ideal for:

Surface Plasmon Resonance (SPR) experiments due to low UV absorbance and excellent buffering stability

Cell culture and biochemical assays, where consistent pH is critical for protein and cellular function

Custom formulations with ions or additives tailored to specific research needs

  • Minimizes pH drift across temperature changes
  • Compatible with optical detection methods due to low UV absorbance
  • Supports protein integrity and cell viability in dynamic systems

Whether you're studying biomolecular interactions, culturing cells, or running high-sensitivity assays, HEPES Buffered Saline offers stability, flexibility, and performance.

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Frequently Asked Questions

HBS is less sensitive to temperature fluctuations than Tris, providing more consistent pH control in experiments where temperature changes may occur.

HBS has low UV absorbance and excellent buffering capacity, which helps prevent signal interference in surface plasmon resonance studies, ensuring accurate biomolecular interaction analysis.

Yes, HBS formulations can be adjusted with calcium, magnesium, or other additives to meet specific assay or cell culture requirements.