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NP-40
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NP-40

NP-40 lysis buffer is a non-ionic detergent-based solution designed for gentle cell lysis while preserving protein complexes and nuclear structure. It’s ideal for applications where complete disruption isn’t necessary, allowing selective solubilization of cytoplasmic and membrane proteins.

  • Provides controlled lysis of cell and organelle membranes without full nuclear disruption
  • Preserves protein-protein interactions for immunoprecipitation and co-IP workflows
  • Ideal for preparing lysates for ELISA, western blotting, and enzyme assays

Whether you're isolating cytoplasmic proteins, studying membrane dynamics, or capturing native protein complexes, our NP-40 lysis buffer offers a precise balance between disruption and preservation.

NP-40

Showing 1–2 of 2 products

NP-40 Lysis Buffer

NP-40 Lysis Buffer

From: $37.00

NP-40 Lysis Buffer (2X)

NP-40 Lysis Buffer (2X)

From: $57.00

FAQs

NP-40 buffer is used for mild cell lysis, allowing extraction of cytoplasmic and some membrane-associated proteins while preserving nuclear and chromatin structure.

NP-40 is gentler than RIPA, making it better suited for extracting proteins while preserving interactions. RIPA is harsher and suited for complete cell lysis and membrane solubilization.

Lysates prepared with NP-40 are compatible with ELISA, western blotting, enzyme activity assays, and protein quantification methods.

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