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Triton X nonionic surfactants

Triton X-100 lysis buffer is a non-ionic detergent-based solution ideal for gentle cell disruption and membrane solubilization. It preserves protein function while effectively breaking open plasma membranes and organelles, making it a staple in protein analysis and subcellular fractionation workflows.

  • Gently lyses cells while preserving protein activity and interactions
  • Solubilizes membranes including endoplasmic reticulum, nuclear envelope, and plasma membrane
  • Ideal for cell fractionation, western blotting, and enzyme activity assays

Whether you're isolating organelles, studying protein complexes, or extracting functional enzymes, our Triton X-100 buffer offers effective lysis with minimal disruption to sensitive biomolecules.

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Frequently Asked Questions

Triton X-100 lysis buffer is used to solubilize cellular membranes and extract proteins without denaturing them, making it ideal for protein studies and subcellular fractionation.

Triton X-100 is milder than SDS and slightly stronger than NP-40. It’s preferred when preserving protein function or enzyme activity is important.

Triton X-100 lysis buffer is compatible with SDS-PAGE, western blotting, enzyme activity assays, and protein quantification workflows.