Phosphate Buffered Saline with Tween-20 (PBST) is a modified PBS solution designed to reduce non-specific binding and enhance signal clarity in immunoassays. By combining PBS with Tween-20, a non-ionic surfactant, PBST improves washing efficiency and helps maintain protein solubility and assay consistency.
With a stable pH range of 7.0–7.4, PBST is ideal for:
ELISA and Western blotting workflows
Washing and blocking steps in antibody-based assays
Reducing background noise for improved signal-to-noise ratio
Minimizes non-specific binding in sensitive detection assays
Maintains ionic strength and protein solubility
Improves assay reproducibility and signal clarity
Whether you're optimizing an immunoassay or standardizing lab protocols, PBST provides the consistency, cleanliness, and performance needed for reliable results.
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Frequently Asked Questions
What is the purpose of adding Tween-20 to PBS?
Tween-20 is a mild surfactant that reduces non-specific protein binding, helping to lower background noise in immunoassays like ELISA and Western blotting.
When should I use PBST instead of regular PBS?
PBST is preferred during washing and blocking steps in assays where high specificity and low background are essential. Regular PBS lacks the detergent necessary to reduce non-specific interactions.
Can PBST interfere with antibody-antigen binding?
No, PBST is formulated to preserve specific binding while minimizing unwanted interactions. It is widely used to improve accuracy and clarity in antibody-based detection systems.
