PBST

Phosphate Buffered Saline with Tween-20 (PBST) is a modified PBS solution designed to reduce non-specific binding and enhance signal clarity in immunoassays. By combining PBS with Tween-20, a non-ionic surfactant, PBST improves washing efficiency and helps maintain protein solubility and assay consistency.

With a stable pH range of 7.0–7.4, PBST is ideal for:

ELISA and Western blotting workflows

Washing and blocking steps in antibody-based assays

Reducing background noise for improved signal-to-noise ratio

• Minimizes non-specific binding in sensitive detection assays
• Maintains ionic strength and protein solubility
• Improves assay reproducibility and signal clarity

Whether you're optimizing an immunoassay or standardizing lab protocols, PBST provides the consistency, cleanliness, and performance needed for reliable results.