Potassium phosphate buffer is a widely used solution in biochemical and molecular biology applications, known for its effective buffering capacity in the pH range of 5.8 to 8.0. It is particularly valued for its compatibility with various biological systems and its ability to maintain stable pH conditions during experiments.
Provides consistent pH conditions essential for enzyme activity and protein stability
Strong buffering capacity with minimal reactivity, making it suitable for sensitive biochemical assays
Highly soluble in water, ensuring easy integration into various laboratory protocols
Whether you're conducting enzyme assays, protein purification, or cell culture experiments, our potassium phosphate buffer ensures reliable performance and reproducible results.
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Frequently Asked Questions
What is potassium phosphate buffer used for?
Potassium phosphate buffer is commonly used in biochemical and molecular biology applications, including enzyme assays, protein purification, and cell culture, due to its effective pH buffering capacity.
Why is potassium phosphate buffer a suitable reagent in blocking buffer for immunolabeling?
Potassium phosphate buffer provides stable pH and ionic conditions that help reduce nonspecific binding during immunolabeling. Its compatibility with proteins and low background reactivity make it an ideal base for blocking buffers in applications like immunohistochemistry and western blotting.
How should potassium phosphate buffer be stored?
Store potassium phosphate buffer at room temperature in a tightly sealed container to maintain its stability and prevent contamination.