Blocking

Blocking buffers are essential for reducing background and improving specificity in western blotting and other membrane-based detection assays. These buffers coat unbound areas of the membrane to prevent non-specific antibody binding, allowing your target signal to stand out clearly and reliably.

  • Minimizes background by preventing non-specific interactions on membranes
  • Enhances antibody binding specificity for improved signal clarity
  • Buffer selection can be optimized based on membrane type and antibody characteristics to improve specificity and reduce background

Whether you're optimizing a western blot or fine-tuning a nucleic acid detection assay, our blocking buffers help ensure clean, reliable results every time.

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Frequently Asked Questions