RIPA buffer is a powerful, all-purpose lysis solution designed to extract proteins from nearly every cellular compartment including nuclear, membrane, and mitochondrial structures. Its ionic and non-ionic detergent blend enables efficient solubilization of total protein from mammalian cells and tissues.
Rapidly disrupts cell membranes for high-yield protein extraction
Solubilizes cytoplasmic, membrane-bound, and nuclear proteins in one step
Ideal for western blotting, immunoprecipitation, ELISA, and SDS-PAGE
Whether you're preparing lysates for quantitative detection or analyzing complex signaling pathways, our RIPA buffer delivers the strength and versatility trusted by molecular biologists.
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Frequently Asked Questions
What is RIPA buffer used for?
RIPA buffer is used to lyse cells and solubilize proteins from cytoplasmic, nuclear, membrane, and mitochondrial compartments for downstream analysis.
What makes RIPA buffer different from milder lysis buffers?
Unlike buffers like NP-40 or Triton X-100, RIPA contains stronger ionic detergents (e.g., SDS, sodium deoxycholate) that provide more complete lysis and extraction, especially for membrane-associated and nuclear proteins.
What applications is RIPA buffer compatible with?
It’s commonly used in SDS-PAGE, western blotting, ELISA, immunoprecipitation, and protein quantification assays like BCA or Bradford.
