Toggle Nav
Frequently Asked Questions
Tris buffer maintains stable pH in the physiological range (7.0–9.0), making it essential for DNA/RNA extraction, protein purification, and electrophoresis techniques like SDS-PAGE.
Dissolve the appropriate amount of Tris base in deionized water, adjust the pH using HCl or NaOH as required, and filter sterilize if necessary. The concentration will depend on your specific application.
Tris has minimal interference with most biomolecules, making it suitable for protein and nucleic acid studies. However, it may affect enzyme activity at high concentrations, so careful optimization is recommended.