Why is Tris buffer widely used in molecular biology?
Tris buffer maintains stable pH in the physiological range (7.0–9.0), making it essential for DNA/RNA extraction, protein purification, and electrophoresis techniques like SDS-PAGE.
How do I prepare Tris buffer for my experiment?
Dissolve the appropriate amount of Tris base in deionized water, adjust the pH using HCl or NaOH as required, and filter sterilize if necessary. The concentration will depend on your specific application.
Does Tris buffer interact with proteins or nucleic acids?
Tris has minimal interference with most biomolecules, making it suitable for protein and nucleic acid studies. However, it may affect enzyme activity at high concentrations, so careful optimization is recommended.
