Phosphate Buffered Saline, PBS (10X, with Triton X-100)
CAT#: BM-220T
- Useful in cell culture and extraction protocols
- Effective in disrupting cell membranes while regulating pH and osmotic pressure
- Ready-to-use once diluted to 1X working concentration
Available sizes:
Product Description
The 10X Phosphate Buffered Saline (PBS) with Triton X-100 is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | PBS, PBS with Triton X-100, Phosphate Buffered Saline |
| pH: | 7.40 ± 0.15 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Sodium Phosphate Dibasic | 7558-79-4 | 141.96 | 101 mM |
| Potassium Phosphate Monobasic | 7778-77-0 | 136.09 | 17.6 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 27 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 1.37 M |
| Triton X-100 | 9036-19-5 | ~625 | 1% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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