Kinase extraction buffer is engineered to efficiently lyse cells and release active protein kinases from both cytoplasmic and nuclear compartments. It preserves phosphorylation states and enzymatic activity, making it ideal for sensitive kinase assays and signaling pathway analysis.
Maximizes recovery of active kinases for downstream functional studies
Maintains phosphorylation integrity for accurate signal transduction analysis
Compatible with kinase assays, western blotting, and inhibitor screening
Whether you're mapping phosphorylation events, profiling drug responses, or investigating signaling networks, our kinase extraction buffer delivers the clean, active lysates your research depends on.
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Frequently Asked Questions
What is kinase extraction buffer used for?
Kinase extraction buffer is used to lyse cells and extract both cytoplasmic and nuclear kinases while preserving their activity and phosphorylation state for downstream analysis.
What types of assays are compatible with kinase extraction buffer?
Common applications include western blotting, ELISA, kinase activity assays, mass spectrometry, and phospho-specific antibody detection.
What are the optimal conditions for kinase extraction?
Kinase extraction should be performed at 4°C using a lysis buffer supplemented with protease and phosphatase inhibitors to preserve enzyme activity and phosphorylation status. Rapid processing and minimal freeze-thaw cycles are also recommended.
