Low salt lysis buffer is specially formulated for gentle yet effective cell disruption, particularly from primary cells, tissue samples, or sensitive cultured cells. With reduced ionic strength and built-in inhibitors, it enables extraction of intact proteins suitable for downstream analysis.
Preserves protein integrity and phosphorylation with reduced salt-induced denaturation
Ideal for lysing delicate cell types without compromising yield or function
Compatible with western blotting, ELISA, IP, PAGE, and protein purification workflows
Whether you're studying native protein interactions or preparing lysates for high-sensitivity detection, our low salt lysis buffer gives you clean results without harsh conditions.
Read More
Frequently Asked Questions
What is low salt lysis buffer used for?
Low salt lysis buffer is used to gently lyse primary cells, tissues, or sensitive cultured cells while preserving protein structure and post-translational modifications for downstream analysis.
Why use a low salt lysis buffer instead of a standard lysis buffer?
Lower ionic strength reduces the risk of protein denaturation, aggregation, or disruption of protein complexes—making it ideal for studying native interactions or extracting fragile proteins.
What applications is this buffer compatible with?
It’s suitable for immunoprecipitation, western blotting, ELISA, polyacrylamide gel electrophoresis, and many purification methods.
