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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Potassium Phosphate
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  5. Potassium Phosphate

Potassium Phosphate

Potassium phosphate buffer is a widely used solution in biochemical and molecular biology applications, known for its effective buffering capacity in the pH range of 5.8 to 8.0. It is particularly valued for its compatibility with various biological systems and its ability to maintain stable pH conditions during experiments.

  • Provides consistent pH conditions essential for enzyme activity and protein stability
  • Strong buffering capacity with minimal reactivity, making it suitable for sensitive biochemical assays
  • Highly soluble in water, ensuring easy integration into various laboratory protocols

Whether you're conducting enzyme assays, protein purification, or cell culture experiments, our potassium phosphate buffer ensures reliable performance and reproducible results.

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Potassium Phosphate

Showing 1–2 of 2 products

Potassium Phosphate Buffer (0.5 M, pH 7.4)

Potassium Phosphate Buffer (0.5 M, pH 7.4)

From: $70.00

Potassium Phosphate Buffer (0.2 M, pH 7.4)

Potassium Phosphate Buffer (0.2 M, pH 7.4)

From: $70.00

FAQs

Potassium phosphate buffer is commonly used in biochemical and molecular biology applications, including enzyme assays, protein purification, and cell culture, due to its effective pH buffering capacity.

Potassium phosphate buffer provides stable pH and ionic conditions that help reduce nonspecific binding during immunolabeling. Its compatibility with proteins and low background reactivity make it an ideal base for blocking buffers in applications like immunohistochemistry and western blotting.

Store potassium phosphate buffer at room temperature in a tightly sealed container to maintain its stability and prevent contamination.