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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Sodium Cacodylate
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  5. Sodium Cacodylate

Sodium Cacodylate

Sodium cacodylate buffer is an aqueous organic compound producing inorganic trivalent arsenic when metabolized with an effective pH buffering capacity in the range of 5.1 to 7.4. It is particularly valued for its compatibility with fixatives and its ability to maintain stable pH conditions during specimen preparation.

  • Chemically stable under electron beam exposure, supporting artifact-free imaging
  • Does not react with common contrast agents, preserving staining integrity
  • Preserves fine cellular and subcellular detail for high-resolution imaging

Whether you're conducting transmission electron microscopy, scanning electron microscopy, or other ultrastructural studies, our sodium cacodylate buffer ensures reliable performance and high-quality results.

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Sodium Cacodylate

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Sodium Cacodylate Buffer (0.1 M, pH 6.5)

Sodium Cacodylate Buffer (0.1 M, pH 6.5)

From: $82.00

FAQs

Sodium cacodylate buffer is commonly used in electron microscopy applications, including transmission and scanning electron microscopy, due to its effective pH buffering capacity.

Sodium cacodylate buffer offers excellent pH stability, compatibility with various fixatives, and minimal interaction with cellular components, making it ideal for electron microscopy.

Sodium cacodylate buffer is widely used in electron microscopy because it maintains a stable pH during fixation, which is essential for preserving cellular ultrastructure. It is also highly compatible with fixatives like glutaraldehyde and osmium tetroxide, helping to reduce artifacts and improve image contrast.