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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Lab scientist preparing standard buffer solutions with precision instruments
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Standard Buffers

Standard buffers are high-quality, pre-formulated solutions designed to maintain stable pH under experimental conditions. They offer consistent composition and are manufactured to deliver reproducible performance from batch to batch.

  • Pre-calibrated for exact pH and ionic strength
  • Ready-to-use to streamline experimental setup
  • Suitable for routine and high-sensitivity applications

Whether you're working on enzyme assays, electrophoresis, or nucleic acid purification, our standard buffers help ensure consistent results every time.

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FAQs

Standard buffers are essential for maintaining stable pH conditions in experiments such as biochemical assays, electrophoresis, and enzyme studies.

Pre-made standard buffers save time, reduce variability, and ensure precise, reliable pH and ionic strength, especially important for sensitive applications.

Choosing the right standard buffer depends on the required pH range, ionic strength, and compatibility with your experimental system. Consider the specific needs of your assay-such as enzyme activity, nucleic acid stability, or protein solubility-and select a buffer that maintains optimal conditions throughout the procedure.