Luminol & Oxidizing Solutions
CAT#: WB-100
- Useful in protein detection and quantification
- Commonly used in immunoblotting procedures to detect HRP-conjugated antibodies
- Ready-to-use oxidizing solution and luminol solution kit
Available sizes:
Product Description
Luminol & Oxidizing Solutions are chemicals used in chemiluminescent detection, a popular and highly sensitive protein detection method for Western Blotting. In this method, the enzyme known as horseradish peroxidase (HRP) conjugated to a secondary antibody reacts with a luminol, a chemiluminescent substrate. In the presence of an Oxidizing Solution, HRP oxidizes luminol to an excited state that emits light at 425 nm.
Specifications
| Storage: | 2-8°C |
|---|---|
| QC Testing: | Appearance |
| Notes: | After secondary antibody incubation, wash the membrane with PBS or TBS four times. 2. Mix equal amount of Luminol solution and Oxidizing solution just before use. 3. Incubate the membrane in this solution for one minute under constant agitation. 4. Expose the X-ray films for different periods of time. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| 3-Aminophtalhydrazide (Luminol) | 521-31-3 | 177.16 | 0.4 mM |
| 4-Iodophenol | 540-38-5 | 220.01 | 8 mM |
| Dimethyl Sulfoxide (DMSO) | 67-68-5 | 78.13 | 10 mL/L |
| Glycine | 56-40-6 | 75.07 | 100 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
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