Tris-Acetate-EDTA Buffer, TAE (50X)
CAT#: BM-250
- Useful in cell culture, biochemistry, and molecular biology techniques
- Effective in nucleic acid separation, biomolecule purification, and pH regulation
- Ready-to-use 50X solution, providing a buffering system within the pH range of 8.1-8.4
Available sizes:
Product Description
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | TAE |
| pH: | 8.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 2 M |
| Acetic Acid, Glacial | 64-19-7 | 60.05 | 1 M |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 50 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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