Tris-Borate-EDTA Buffer, TBE (10X)

CAT#: BM-260
  • Useful in biochemistry and molecular biology techniques
  • Effective as a pH regulator in nucleic acid electrophoresis to separate DNA/RNA fragments based on size
  • Ready-to-use solution, providing a buffering system within the pH range of 8.1-8.5

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CAT#:
BP-177-500ml Before requesting a quote or making a purchase, please make sure you select the correct size.
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Product Description

The Tris-Borate-EDTA (TBE) Buffer is a pH regulator and chelator used in molecular biology techniques such as nucleic acid gel electrophoresis. Supporting a pH range between 8.1 and 8.5, the TBE Buffer combines aqueous tris base, boric acid, and EDTA (Ethylenediaminetetraacetic Acid), making it efficient as a running buffer or component of agarose gels. This product is prepared in a 10X stock concentration, and can be diluted to concentrations ideal to specific laboratory workflows.

Specifications

More Information
Method: Filtered through 0.22-micron filter.
Storage: Room Temp.
Synonym: TBE
pH: 8.30 ± 0.20
QC Testing: Appearance, pH
Notes: To make 1X, dilute 10-fold with deionized water.
Component NameCASMolecular WeightConcentration
Tris-Base 77-86-1 121.14 890 mM
Boric Acid 10043-35-3 61.83 890 mM
EDTA Disodium Salt Dihydrate 6381-92-6 372.24 20 mM
Water 7732-18-5 18.02 Rest