Tris-Borate-EDTA Buffer, TBE (10X)
CAT#: BM-260
- Useful in biochemistry and molecular biology techniques
- Effective as a pH regulator in nucleic acid electrophoresis to separate DNA/RNA fragments based on size
- Ready-to-use solution, providing a buffering system within the pH range of 8.1-8.5
Available sizes:
Product Description
The Tris-Borate-EDTA (TBE) Buffer is a pH regulator and chelator used in molecular biology techniques such as nucleic acid gel electrophoresis. Supporting a pH range between 8.1 and 8.5, the TBE Buffer combines aqueous tris base, boric acid, and EDTA (Ethylenediaminetetraacetic Acid), making it efficient as a running buffer or component of agarose gels. This product is prepared in a 10X stock concentration, and can be diluted to concentrations ideal to specific laboratory workflows.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | TBE |
| pH: | 8.30 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 10-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 890 mM |
| Boric Acid | 10043-35-3 | 61.83 | 890 mM |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 20 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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