Bis-Tris Transfer Buffer (20X)
CAT#: BP-193
- Useful in electrophoresis and blotting techniques
- Commonly used for the transfer of proteins from polyacrylamide gels to membranes
- Ready-to-use once diluted to 1X working concentration
Available sizes:
Product Description
The 20X Bis-Tris Transfer Buffer is often used to transfer proteins from Bis-Tris and Tris-Acetate gels to nitrocellulose or PVDF membranes for Western blotting. It is useful as it maintains a neutral environmental pH during transfer protocols, which is suitable for most protein types, crucial for structural preservation of proteins, and effective at minimizing protein denaturation.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 7.30 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 20-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Bis-Tris | 6976-37-0 | 209.24 | 500 mM |
| Bicine | 150-25-4 | 163.17 | 500 mM |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 20 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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