Kinase Extraction Buffer
CAT#: BP-116K
- Useful in cell lysis and extraction protocols
- Effective in releasing cytoplasmic and nuclear kinases for protein research
- Ready-to-use solution, providing a buffering system within the pH range of 7.0-8.0
Available sizes:
Product Description
The Kinase Extraction Buffer is a high-performance solution designed to efficiently extract and stabilize protein kinases from various biological samples. It lyses cell membranes and nuclear envelopes to release cytoplasmic and nuclear kinases, ensuring optimal conditions for kinase assays. Ideal for research in signal transduction, cancer biology, and drug discovery, this buffer supports the study of kinase interactions, phosphorylation events, and inhibitor screening.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | Kinase Assay Buffer, Protein Kinase Lysis Buffer |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.12 / 157.6 | 20 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 100 mM |
| Sodium Fluoride | 7681-49-4 | 41.99 | 50 mM |
| Beta Glycerophosphate | 154804-51-0 | 216.04 | 10 mM |
| Sodium Deoxycholate | 302-95-4 | 414.55 | 0.1% (w/v) |
| Sodium Dodecyl Sulfate (SDS) | 151-21-3 | 288.38 | 0.1% (w/v) |
| EDTA | 60-00-4 | 292.24 | 5 mM |
| Triton X-100 | 9036-19-5 | ~625 | 1% (v/v) |
| Glycerol | 56-81-5 | 92.09 | 10% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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