CAPS Buffer (0.5 M, pH 11.0)
CAT#: BB-74
- Useful in protein research, purification, biochemistry, and molecular biology
- Enhances protein stability while regulating environmental pH
- Ready-to-use solution, providing a buffering system within the pH range of 9.7-11.1
Available sizes:
Product Description
The CAPS buffer, or 3-(cyclohexylamino)-1-propanesulfonic acid buffer, is a zwitterionic Good's buffer commonly used to maintain pH levels within the range of 9.7 to 11.1. This buffers support several applications including protein crystallization, enzymatic reactions, and western and immunoblotting experiments. It is an excellent buffer for the transfer of proteins with high molecular weights to nitrocellulose membranes after SDS-PAGE. CAPS buffer can also be used in protein sequencing.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | CAPS |
| pH: | 11.00 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| CAPS | 1135-40-6 | 221.32 | 500 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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CAPS Buffer (0.5 M, pH 10.0)- BB-72Sku#: BB-72