The 6X laemmli sample buffer is a critical component in the preparation of protein samples for SDS-PAGE, ensuring the resolution of protein bands. Its composition typically includes Tris-HCl, glycerol, SDS, and a tracking dye like Bromophenol Blue. In the reducing Laemmli sample buffer, a reducing agent such as β-mercaptoethanol or dithiothreitol (DTT) is added to break disulfide bonds within protein subunits, allowing them to separate by size in electrophoresis.
The product can be solidified at low temperature; please warm up the product to bring back all content to the solution; this will not impact the product integrity.