Laemmli SDS-Sample Buffer (6X, Reducing)
CAT#: BP-111R
- Useful in biochemistry and molecular biology techniques
- Commonly used to fully denature proteins into linearized chains for SDS-PAGE protocols
- Ready-to-use once diluted to 1X working concentration
Available sizes:
Product Description
The 6X laemmli sample buffer is a critical component in the preparation of protein samples for SDS-PAGE, ensuring the resolution of protein bands. Its composition typically includes Tris-HCl, glycerol, SDS, and a tracking dye like Bromophenol Blue. In the reducing Laemmli sample buffer, a reducing agent such as β-mercaptoethanol or dithiothreitol (DTT) is added to break disulfide bonds within protein subunits, allowing them to separate by size in electrophoresis.
Specifications
| Storage: | > 25 °C |
|---|---|
| pH: | 6.80 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | The product can be solidified at low temperature; please warm up the product to bring back all content to the solution; this will not impact the product integrity. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 375 mM |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 9% (w/v) |
| Glycerol | 56-81-5 | 92.09 | 50% (v/v) |
| Bromophenol Blue | 34725-61-6 | 691.94 | 0.075% (w/v) |
| 2-Mercaptoethanol | 60-24-2 | 78.13 | 9% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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