Transfer Buffer (10X, Electro Blotting)
CAT#: BP-190
- Useful in biochemistry and molecular biology techniques
- Facilitate the transfer of proteins from SDS-PAGE gels to membranes when diluted to 1X
- Ready-to-use once diluted to 1X working concentration
Available sizes:
Product Description
The 10X Transfer Buffer-Electro Blotting is the most commonly used buffer in the wet transfer system for the electrotransfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. The addition of 10% methanol in this buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 8.50 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make this a 1X solution, dilute 10-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 250 mM |
| Glycine | 56-40-6 | 75.07 | 1.92 M |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
Powered by BiozRelated Products
-
Bis-Tris Transfer Buffer (20X)- BP-193Sku#: BP-193
-
Semi Dry Blot Transfer Buffer (10X)- BP-194Sku#: BP-194