Triton X-100 Lysis Buffer
CAT#: BP-117
- Useful in cell lysis and extraction protocols
- Effective in protein and lipid solubilization
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The Triton X-100 Lysis Buffer is a widely used lysis buffer in molecular biology and biochemistry applications. Composed of buffering components as well as Triton X-100, a nonionic surfactant, this lysis buffer solubilizes lipids and proteins, effectively breaking open cellular compartments. The Triton X-100 Lysis buffer is known for its effectiveness in cell fractionation extraction protocols, due to its ability to solubilize the endoplasmic reticulum, plasma membrane, and nuclear envelope.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | For long term storage, store product at -20°C. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 50 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
| Triton X-100 | 9036-19-5 | ~625 | 1% |
| EDTA (0.5 M, pH 7.4) | 60-00-4 | 292.24 | 5 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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