Krebs-Henseleit Buffer (with HEPES, without Calcium)
CAT#: BSS-265W
- Useful in cell and tissue culture and preservation
- Recommended Shelf Life of 6 months
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The Krebs-Henseleit buffer with HEPES and without Calcium is a balanced salt solution used in cell culture and tissue preservation to maintain physiological pH and osmotic balance, and is formulated at a pH of 7.40 ± 0.15. It contains glucose, magnesium sulfate, potassium phosphate, potassium chloride, sodium chloride, sodium bicarbonate and HEPES. This buffer is ideal for experiments requiring stable pH in with environments not requiring calcium.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | Krebs-Henseleit Solution |
| pH: | 7.40 ± 0.15 |
| QC Testing: | Appearance, pH |
| Notes: | This product was formerly listed as PY-946W. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Sodium Chloride | 7647-14-5 | 58.44 | 118 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 4.7 mM |
| Magnesium Sulfate Anhydrous | 7487-88-9 | 120.37 | 1.2 mM |
| Potassium Phosphate Monobasic Anhydrous | 7778-77-0 | 136.09 | 1.2 mM |
| Sodium Bicarbonate | 144-55-8 | 84.01 | 25 mM |
| HEPES | 7365-45-9 | 238.3 | 10 mM |
| Dextrose | 50-99-7 | 180.16 | 11.1 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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