MOPS-SDS Running Buffer (20X)
CAT#: BP-178
- Useful in biochemistry and molecular biology techniques
- Often used as a running buffer in SDS-PAGE to separate proteins based on their molecular weight
- Ready-to-use once diluted to 1X working concentration
Available sizes:
Product Description
The 20X MOPS-SDS Running Buffer is generally used for resolving/separating medium to large-sized proteins on Bis-Tris gels under denaturing conditions in SDS-PAGE. When reconstituted from the 20X concentrate, this buffer maintains a stable pH of approximately 7.7. Use of MOPS-SDS Running Buffer allows proteins to run slower which might prove ideal for high-resolution of proteins on SDS-PAGE.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | MOPS |
| pH: | 7.70 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 20-fold with distilled water. Slight coloration with time will not affect the quality of this product. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| MOPS, Free Acid | 1132-61-2 | 209.26 | 1 M |
| Tris-Base | 77-86-1 | 121.14 | 1 M |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 2% (w/v) |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 20 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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