Tris-Glycine-Native Running Buffer (10X)
CAT#: BP-160
- Useful in biochemistry and molecular biology techniques
- Effective as a running buffer for native polyacrylamide gel electrophoresis
- Ready-to-use once diluted to 1X working concentration
Available sizes:
Product Description
Tris-Glycine Native Running Buffer is most commonly used for protein gel electrophoresis under native running conditions on Tris-Glycine gels or Tris-Acetate gels. It is provided as 10X concentrate, and upon dilution to 1X this buffer maintains a pH at around 8.3. The Tris-Glycine Native Running buffer system helps in maintaining native protein conformation, subunit interactions, and biological activity.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 8.50 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 10-fold with deionized water. Slight coloration with time will not affect the quality of this product. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 250 mM |
| Glycine | 56-40-6 | 75.07 | 1.92 M |
| Water | 7732-18-5 | 18.02 | Rest |