Stripping Buffer (4X, Strong)

CAT#: BP-96
  • Useful in biochemistry and molecular biology techniques
  • Can be used to strip primary and secondary antibodies from Western blot membranes
  • Ready-to-use solution tested for pH

Available sizes:

CAT#:
BP-177-500ml Before requesting a quote or making a purchase, please make sure you select the correct size.
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Product Description

The 4X Stripping buffer (strong) contains about 8% SDS, and is considered strong due to its ability to break antibody-antigen interactions and promote the removal of primary and secondary antibodies from Western blot membranes. This solution enables reprobing Western blot membranes several times with different antibodies, preventing the need for running multiple gels and transfers.

Specifications

More Information
Method: Filtered through 1-micron filter.
Storage: Room Temp.
pH: 6.80 ± 0.20 (1X)
QC Testing: Appearance, pH
Notes: Dilute 4X and add Beta ME (0.6%). Wash the wet membrane in the stripping buffer prior to incubating the membrane for 30 minutes at 65°C under agitation. Following incubation, again wash the membrane in the stripping buffer.
Component NameCASMolecular WeightConcentration
Tris-Base / Tris-HCl 77-86-1 / 1185-53-1 121.14 / 157.60 250 mM
Sodium Dodecyl Sulfate (SDS) [20% Solution] 151-21-3 288.38 8%
Water 7732-18-5 18.02 Rest