Stripping Buffer (4X, Strong)
CAT#: BP-96
- Useful in biochemistry and molecular biology techniques
- Can be used to strip primary and secondary antibodies from Western blot membranes
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The 4X Stripping buffer (strong) contains about 8% SDS, and is considered strong due to its ability to break antibody-antigen interactions and promote the removal of primary and secondary antibodies from Western blot membranes. This solution enables reprobing Western blot membranes several times with different antibodies, preventing the need for running multiple gels and transfers.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 6.80 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | Dilute 4X and add Beta ME (0.6%). Wash the wet membrane in the stripping buffer prior to incubating the membrane for 30 minutes at 65°C under agitation. Following incubation, again wash the membrane in the stripping buffer. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 250 mM |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 8% |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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