Stripping Buffer (4X, Mild)
CAT#: BP-97
- Useful in biochemistry and molecular biology techniques
- Can be used to strip antibodies from Western blot membranes without removing or damaging the target proteins
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The 4X Stripping Buffer (Mild) containing 200 mM Glycine, is used for breaking antibody-antigen interactions, resulting in removal of primary and secondary antibodies from their target proteins on probed Western blot membranes. The use of this buffer minimizes protein denaturation, preserves epitope integrity, and is suitable for re-probing, saving time without the need for running multiple gels and performing transfers.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 2.50 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | BP-97 is a mild stripping buffer. End user should optimize the conditions for the optimal stripping. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Glycine | 56-40-6 | 75.07 | 200 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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