Stripping Buffer (4X, Mild)

CAT#: BP-97
  • Useful in biochemistry and molecular biology techniques
  • Can be used to strip antibodies from Western blot membranes without removing or damaging the target proteins
  • Ready-to-use solution tested for pH

Available sizes:

CAT#:
BP-177-500ml Before requesting a quote or making a purchase, please make sure you select the correct size.
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Product Description

The 4X Stripping Buffer (Mild) containing 200 mM Glycine, is used for breaking antibody-antigen interactions, resulting in removal of primary and secondary antibodies from their target proteins on probed Western blot membranes. The use of this buffer minimizes protein denaturation, preserves epitope integrity, and is suitable for re-probing, saving time without the need for running multiple gels and performing transfers.

Specifications

More Information
Method: Filtered through 1-micron filter.
Storage: Room Temp.
pH: 2.50 ± 0.20 (1X)
QC Testing: Appearance, pH
Notes: BP-97 is a mild stripping buffer. End user should optimize the conditions for the optimal stripping.
Component NameCASMolecular WeightConcentration
Glycine 56-40-6 75.07 200 mM
Water 7732-18-5 18.02 Rest