Separating (Resolving) Buffer
CAT#: BP-90
- Useful in biochemistry and molecular biology techniques
- Can be used to prepare the resolving gels in SDS-PAGE protocols
- Provided at 4X concentrate and is ready-to-use once diluted to a 1X working concentration
Available sizes:
Product Description
The Separating (Resolving) Buffer is a critical component in SDS-Polyacrylamide Gel Electrophoresis (PAGE), a laboratory technique that is used to separate protein molecules based on molecular weight. This buffer maintains the pH of the separating or resolving gel at 8.8, which is a higher pH level than that of the stacking gel.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 8.80 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 1.5 M |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.40% |
| Water | 7732-18-5 | 18.02 | Rest |
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Stacking Buffer- BP-95Sku#: BP-95