HEPES Buffer (0.5 M, pH 7.4, pH Adjusted with KOH)
CAT#: BB-2076-K
- Useful in protein research, purification, biochemistry, and molecular biology
- Supports cell culture, purification and enzyme studies, isoelectric focusing, bioprocessing protocols, and live cell imaging
- Ready-to-use solution, providing a buffering system within the pH range of 6.8-8.2
Available sizes:
Product Description
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This formulation is adjusted with KOH.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | HEPES |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | Slight coloration with time will not affect the quality of this product. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.3 | 500 mM |
| Potassium Hydroxide | 1310-58-3 | 56.11 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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