Tris Buffered Saline, TBS (20X)
CAT#: BM-300X
- Useful in cell culture, molecular biology, protein research, and purification
- Often used as a wash buffer, storage buffer, and for resuspension of cells
- Ready-to-use 20X solution, providing a buffering system within the pH range of 7.4-8.0
Available sizes:
Product Description
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | TBS |
| pH: | 7.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 20-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 500 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 54 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 2.74 M |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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