Tris-EDTA Buffer, TE (20X, pH 8.0)
CAT#: BM-304
- Useful in biochemistry and molecular biology techniques
- Effective in nucleic acid storage, extraction, and within PCR and sequencing techniques
- Ready-to-use 20X solution, providing a buffering system within the pH range of 7.5-8.5
Available sizes:
Product Description
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of a 20X concentration of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allows pH regulation within the range of 7.5-8.5. It is widely used often for DNA and RNA gel electrophoresis, and also for DNA automated sequencing.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | TE |
| pH: | 8.00 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 200 mM |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 20 mM |
| Hydrochloric Acid (6 N) | 7647-01-0 | 36.46 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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