EBC Lysis Buffer
CAT#: BP-1410
- Useful in cell lysis and extraction protocols
- Effective in the removal of red blood cells while preserving target biomolecules
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The EBC Lysis Buffer (Erythrocyte Blood Cell Lysis Buffer) is a buffered detergent solution useful for erythrocyte lysate preparation. Formulated at a pH of 8.0, this solution is buffered with Tris-Base and Tris-HCl, and is also composed of EDTA and NP-40 (IGEPAL CA-630), allowing for the effective lysis of blood cells while preserving nucleic acids and proteins during the process. This reagent is effective in a variety of protocols involving cell culture, protein extraction, immunoprecipitation, enzymatic assays, and nucleic acid isolation.
Specifications
| Method: | Filtered through 0.22 micron filter. |
|---|---|
| Storage: | 2-8°C |
| pH: | 8.00 ± 0.15 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-52-1 | 121.4 / 157.60 | 50 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 120 mM |
| Nonidet P-40 (IGEPAL CA-630) | 9002-93-1 | N/A | 0.5% |
| EDTA | 60-00-4 | 292.24 | 5 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Related Products
-
NP-40 Lysis Buffer (2X)- BP-119XSku#: BP-119X
-
RIPA Buffer (2X)- BP-115XSku#: BP-115X
-
RIPA Buffer (5X)- BP-115-5XSku#: BP-115-5X
-
RIPA Buffer (with EDTA & EGTA)- BP-115DGSku#: BP-115DG
-
RIPA Buffer (with EDTA)- BP-115DSku#: BP-115D
-
WBC Lysis Buffer- IBB-199Sku#: IBB-199
-
NP-40 Lysis Buffer- BP-119Sku#: BP-119