TAPS Buffer (0.2 M, pH 8.5)
CAT#: BB-2372
- Useful in protein research, purification, biochemistry, and molecular biology
- Supports enzyme research, electrophoresis and chromatography techniques, and pH regulation
- Ready-to-use solution, providing a buffering system within the pH range of 7.7-9.1
Available sizes:
Product Description
The TAPS buffer, or N- [Tris (hydroxymethyl)methyl]-3-aminopropanesulfonic acid, is a zwitterionic "Good's" buffer commonly used in molecular biology for its ability to maintain a stable pH. With a pKa of approximately 8.44, TAPS is effective in the pH range of 7.7 to 9.1, making it suitable for capillary electrophoresis to analyze DNA, chromatography techniques to separate dyes, in cell culture media, as enzyme assay buffer, and many other experiments that require a moderately alkaline pH.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | TAPS |
| pH: | 8.50 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| TAPS | 29915-38-6 | 243.28 | 200 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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TAPS Buffer (1 M, pH 8.5)- BB-2375Sku#: BB-2375