Tris Buffered Saline, TBS (10X with 1% Triton X-100)
CAT#: IH-440
- Commonly used in biochemical and molecular biology applications, particularly for immunoassays and Western blotting.
- This buffer helps in washing steps by reducing non-specific binding and improving antibody-antigen interactions.
- 10X buffer can be diluted to 1X for application in immunostaining and western blotting.
Available sizes:
Product Description
TBS (Tris-buffered saline) buffer with Triton is commonly used in biochemical and molecular biology applications, particularly for immunoassays and Western blotting. TBS consists of Tris-HCl and NaCl, maintaining a stable pH, typically around 7.4. The addition of Triton X-100, a non-ionic surfactant, enhances permeability by disrupting lipid membranes, making it useful for cell lysis, protein extraction, and immunostaining. This buffer helps in washing steps by reducing non-specific binding and improving antibody-antigen interactions.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 7.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 500 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 1.5 M |
| Triton X-100 | 9036-19-5 | ~625 | 1% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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Sodium Phosphate Buffer (20 mM, pH 6.5, HPCE Grade)- BBP-65HPCESku#: BBP-65HPCE
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HBS-N Buffer (10X, pH 7.4)- HBS-131Sku#: HBS-131
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HBS-N Buffer (1X, pH 7.4)- HBS-130Sku#: HBS-130
