Tris-HCl Buffer (1 M, pH 9.0)
CAT#: BPS-309
- For use in bioprocessing and biomolecule purification procedures
- Buffering agent capable of regulating bioprocess environments at a pH of 9
- Formulated with USP Compendial-Grade Raw Materials and tested for pH, Conductivity, Osmolality, Endotoxin, DNase, RNase, Protease
Available sizes:
Product Description
The Tris-HCl Buffer is a widely used reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining. This formulation of Tris-HCl is made with WFI-Quality water and is ideal for bioprocessing protocols.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | C₄H₁₁NO₃ · HCl (aq), Tris Hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride |
| pH: | 9.00 ± 0.20 |
| QC Testing: | Appearance, pH, Conductivity, Osmolality, Low Endotoxin, DNase Free, RNase Free, Protease Free |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 1 M |
| Water for Injection (WFI) | 7732-18-5 | 18.02 | Rest |
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