Antibody-antigen docking: specificity lies in the details A variety of common analytical methods relies on antibody-antigen interactions to highlight the presence of specific proteins in biological tissues. However, lengthy, multistep experiments have higher risks of generating false-positive and negative outcomes, resulting in inaccurate data. Preparatory conditions, including the choice of buffers and reagents, influence the antibody's ability to reach and...
High-resolution separation of complex protein mixtures Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is frequently used to obtain high-resolution separation of complex protein mixtures. However, poor band separation can occur due to issues at multiple steps, such as improper sample preparation or insufficient buffering capacity during gel electrophoresis. Complete lysis, improving protein solubility, and quality buffers can help deliver the clearly-resolved...
Consistent, Specific Protein Detection Detect the presence of specific proteins in biological samples, measure changes in protein size, activation state, or posttranslational modifications, or investigate protein-protein interactions. Consistent Western blot success is possible. However, it is threatened by a variety of factors such as variability in sample preparation, inconsistent antibody performance, and the presence of background signal. Buffers are essential...
Improve separation and protein quality with optimized FPLC buffers If you perform biochemical assays on purified proteins, you've likely experienced the pains of FPLC purification. The process separates proteins from whole-cell lysates through differences in size or affinity of the protein using a mobile and stationary phase. Have you experienced insufficient separation? Are your purified proteins inactive or misfolded? This...
Seamless material flow and control All our products are manufactured in-house at our ISO-certified 115,000+ ft2 commercial and manufacturing facility in Milford, MA. Our facility is built to support one continuous, circular process flow. This means each material moves through our facility in a logical, trackable manner - from entering our facility, to being processed, used, filtered, tested, packaged...
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Quality Assurance - We Take It Seriously As a small family business and the true manufacturer of our buffers and solutions, we never sacrifice the quality of our products and services. Whether you are researching biological systems or developing the next generation of therapeutics, you'll set a solid foundation for your science with our products and services. How we...
Terms and Conditions This document represents the most recent version of Boston BioProducts' Terms and Conditions relating to Purchase Orders for products of a custom nature (hereinafter referred to as "Terms and Conditions"). Purchaser accepts these Terms and Conditions at the time a custom order is placed. Such terms and conditions contained within this document will apply to all subsequent...
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