Get strong, clear interaction between antigen and antibody ELISA protocols are generally simple and rewarding to carry out. But a weak signal resulting from not obtaining specific and highly sensitive interaction between the antigen-antibody complex is challenging to deal with. The ELISA buffers you use are the x-factor in the success of running ELISAs, which is where we come in...
Help your cells thrive with well-balanced buffers A cell culture buffer must provide a stable and enriching home away from home for cells to thrive. When a buffer does not offer a precise balance of nutrients, pH, and osmotic pressure, it compromises the cells' growth and development. Don't let a buffer put a barrier between you and the cells' ability...
Isolate plasmid DNA at high yield and purity with buffers and reagents you can trust If you are a molecular biologist who performs cloning, PCR, DNA sequencing, restriction enzyme digestion, transfection, and gene therapy, you've likely needed to isolate plasmid DNA. Are you experiencing low recovery or the presence of impurities? Your plasmid DNA isolation issues may be coming from...
Experience precise separation on your HPLC with quality buffers Whether you use HPLC for analytics or as a means to purify compounds for downstream assays, you've likely experienced issues with your chromatograms. Is your baseline rising? Experiencing low sensitivity? Spikes or noise attributing to poorly resolved bands? This can be attributed to your buffer conditions or quality. See how we...
Antibody-antigen docking: specificity lies in the details A variety of common analytical methods relies on antibody-antigen interactions to highlight the presence of specific proteins in biological tissues. However, lengthy, multistep experiments have higher risks of generating false-positive and negative outcomes, resulting in inaccurate data. Preparatory conditions, including the choice of buffers and reagents, influence the antibody's ability to reach and...
High-resolution separation of complex protein mixtures Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is frequently used to obtain high-resolution separation of complex protein mixtures. However, poor band separation can occur due to issues at multiple steps, such as improper sample preparation or insufficient buffering capacity during gel electrophoresis. Complete lysis, improving protein solubility, and quality buffers can help deliver the clearly-resolved...
Consistent, Specific Protein Detection Detect the presence of specific proteins in biological samples, measure changes in protein size, activation state, or posttranslational modifications, or investigate protein-protein interactions. Consistent Western blot success is possible. However, it is threatened by a variety of factors such as variability in sample preparation, inconsistent antibody performance, and the presence of background signal. Buffers are essential...
Improve separation and protein quality with optimized FPLC buffers If you perform biochemical assays on purified proteins, you've likely experienced the pains of FPLC purification. The process separates proteins from whole-cell lysates through differences in size or affinity of the protein using a mobile and stationary phase. Have you experienced insufficient separation? Are your purified proteins inactive or misfolded? This...
Seamless material flow and control All our products are manufactured in-house at our ISO-certified 115,000+ ft2 commercial and manufacturing facility in Milford, MA. Our facility is built to support one continuous, circular process flow. This means each material moves through our facility in a logical, trackable manner - from entering our facility, to being processed, used, filtered, tested, packaged...
Order Support We want you to get your materials as early as possible. That's why we make our ordering process easy and flexible. We are committed to understanding our current and future customers' needs, meeting their requirements, and exceeding their expectations through exemplary customer satisfaction and attentive customer relations. Accepted Payment We accept check, credit card, and wire payments...
